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KMID : 0903520050480040335
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 4 p.335 ~ p.338
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Qualitative PCR Detection of GM rices (Milyang 204 and Iksan 483) developed in Korea
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Kim Jae-Hwan
Kim Dong-Hern
Ryu Tae-Hoon
Kim Hae-Yeong
Song Hee-Sung
Jee Sang-Mi
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Abstract
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For the development of qualitative PCR detection method of genetically modified rice (Oryza sativa L.), rice species specific gene, SAMDC1 (S-adenosylmethionine decarboxylase), was selected and validated as suitable for use as an endogenous reference gene in rice. The primer pair OsSAMDC1-5’/3’ with 110 bp amplicon was used for amplification of the rice endogenous gene, SAMDC1 and no amplified product was observed from 19 different plants as templates. Qualitative PCR method was assayed with 2 different GM rices (Milyang 204 and Iksan 483) developed in Korea. For the qualitative PCRs, the construct-specific detection primer pairs were constructed. Os204-5’/OsNOS-3’ amplifying the junction region of GUS gene and NOS terminator introduced in Milyang 204 gave rise to an amplicon 172 bp; also, Os483-5’/OsNOS-3’ amplifying the junction region of Bar gene and NOS terminator introduced in Iksan 483 gave rise to an amplicon 161 bp.
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KEYWORD
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GM Rice, Milyang 204, Iksan 483, SAMDC1, PCR
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